Inducible H1 Promoter-Driven Lentiviral siRNA Expression by Stuffer Reporter Deletion
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چکیده
منابع مشابه
Efficient Construction of an Inverted Minimal H1 Promoter Driven siRNA Expression Cassette: Facilitation of Promoter and siRNA Sequence Exchange
BACKGROUND RNA interference (RNAi), mediated by small interfering RNA (siRNA), is an effective method used to silence gene expression at the post-transcriptional level. Upon introduction into target cells, siRNAs incorporate into the RNA-induced silencing complex (RISC). The antisense strand of the siRNA duplex then "guides" the RISC to the homologous mRNA, leading to target degradation and gen...
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ژورنال
عنوان ژورنال: Oligonucleotides
سال: 2005
ISSN: 1545-4576,1557-8526
DOI: 10.1089/oli.2005.15.139